Original Article
Volume: 3 | Issue: 2 | Published: Sep 09, 2020 | Pages: 71 - 76 | DOI: 10.24911/JBCGenetics/183-1588174375
Association of vitamin D receptor gene fok1 polymorphism with bone health in Pakistani population
Authors: Najam Farooq , Anwar Ullah , Abida Arshad , Navida Riaz , Jabar Zaman Khan , Sobia Tabassum , Muhammad Arshad Malik
Article Info
Authors
Najam Farooq
Department of Biological Sciences, International Islamic University Islamabad, Pakistan
Anwar Ullah
Rehman Medical Institute Hayatabad Peshawar, Pakistan
Abida Arshad
Department of Zoology, PMAS Arid Agriculture Rawalpindi Pakistan
Navida Riaz
Department of Biological Sciences, International Islamic University Islamabad, Pakistan
Jabar Zaman Khan
Department of Biological Sciences, International Islamic University Islamabad, Pakistan
Sobia Tabassum
Department of Biological Sciences, International Islamic University Islamabad, Pakistan
Muhammad Arshad Malik
Department of Biological Sciences, International Islamic University Islamabad, Pakistan
Publication History
Received: May 04, 2020
Accepted: July 03, 2020
Published: September 09, 2020
Abstract
Background: Osteoporosis is the bone disease characterized by demineralization of the bone. One of the most important genetic factors responsible for the osteoporosis is the vitamin D receptor (VDR) gene polymorphism. A translocation polymorphism which changes the codon from ATG to ACG has been associated with bone mineral density (BMD) variation and severity of the disease in patients of osteoporosis. The objective of the study was to find association of VDR Fok1 polymorphism with bone mineral density Methods: This case control study was design to find the association of the VDR Fok1 polymorphism with bone health in Pakistani osteoporotic patients. The study was conducted at Islamabad Diagnostic center from 2014 to July 2016. Total of 156 participant (osteoporatic patients n = 78 and normal health controls n = 78 case control) were enrolled in the study. Polymerase chain Reaction restriction length polymorphism (PCR-RFLP) was used to genotype VDR Fok1 polymorphism. Bidirectional sanger sequencing was used to verify the PCR-RFLP results with randomly picked n = 10 samples from both controls and patients. Commercial kits were used to estimate serum calcium and vitamin D level while dual-energy X-ray absorptiometry scan was used to measure the bone mineral density. The data were analyzed statistically using Statistical package for the social sciences (SPSS). Result: F-allele increased the risk for decrease bone mineral density and osteopenia nearly threefold [Odds Ratio (OR): 2.8; 95% Confidence Interval (CI): 3.2-19.0; p ≤ 0.001]. The genotype frequencies (Ff + ff vs. FF) showed an increase risk of disease (OR = 6.79; 95% CI = 3.41-22.6; p =
Keywords: VDR, Fok1, BMD, osteoporosis
Pubmed Style
Najam Farooq, Anwar Ullah, Abida Arshad, Navida Riaz, Jabar Zaman Khan, Sobia Tabassum, Muhammad Arshad Malik. Association of vitamin D receptor gene fok1 polymorphism with bone health in Pakistani population. JBC Genetics. 2020; 09 (September 2020): 71-76. doi:10.24911/JBCGenetics/183-1588174375
Web Style
Najam Farooq, Anwar Ullah, Abida Arshad, Navida Riaz, Jabar Zaman Khan, Sobia Tabassum, Muhammad Arshad Malik. Association of vitamin D receptor gene fok1 polymorphism with bone health in Pakistani population. https://www.jbcgenetics.com/index.php/articles/2156 [Access: April 27, 2025]. doi:10.24911/JBCGenetics/183-1588174375
AMA (American Medical Association) Style
Najam Farooq, Anwar Ullah, Abida Arshad, Navida Riaz, Jabar Zaman Khan, Sobia Tabassum, Muhammad Arshad Malik. Association of vitamin D receptor gene fok1 polymorphism with bone health in Pakistani population. JBC Genetics. 2020; 09 (September 2020): 71-76. doi:10.24911/JBCGenetics/183-1588174375
Vancouver/ICMJE Style
Najam Farooq, Anwar Ullah, Abida Arshad, Navida Riaz, Jabar Zaman Khan, Sobia Tabassum, Muhammad Arshad Malik. Association of vitamin D receptor gene fok1 polymorphism with bone health in Pakistani population. JBC Genetics. (2020), [cited April 27, 2025]; 09 (September 2020): 71-76. doi:10.24911/JBCGenetics/183-1588174375
Harvard Style
Najam Farooq, Anwar Ullah, Abida Arshad, Navida Riaz, Jabar Zaman Khan, Sobia Tabassum, Muhammad Arshad Malik (2020) Association of vitamin D receptor gene fok1 polymorphism with bone health in Pakistani population. JBC Genetics, 09 (September 2020): 71-76. doi:10.24911/JBCGenetics/183-1588174375
Chicago Style
Najam Farooq, Anwar Ullah, Abida Arshad, Navida Riaz, Jabar Zaman Khan, Sobia Tabassum, Muhammad Arshad Malik. "Association of vitamin D receptor gene fok1 polymorphism with bone health in Pakistani population." 09 (2020), 71-76. doi:10.24911/JBCGenetics/183-1588174375
MLA (The Modern Language Association) Style
Najam Farooq, Anwar Ullah, Abida Arshad, Navida Riaz, Jabar Zaman Khan, Sobia Tabassum, Muhammad Arshad Malik. "Association of vitamin D receptor gene fok1 polymorphism with bone health in Pakistani population." 09.September 2020 (2020), 71-76. Print. doi:10.24911/JBCGenetics/183-1588174375
APA (American Psychological Association) Style
Najam Farooq, Anwar Ullah, Abida Arshad, Navida Riaz, Jabar Zaman Khan, Sobia Tabassum, Muhammad Arshad Malik (2020) Association of vitamin D receptor gene fok1 polymorphism with bone health in Pakistani population. , 09 (September 2020), 71-76. doi:10.24911/JBCGenetics/183-1588174375