Original Article
Volume: 3 | Issue: 1 | Published: May 14, 2020 | Pages: 3 - 6 | DOI: 10.24911/JBCGenetics/183-1582098144
Association of vitamin D level and CYP27B1 gene polymorphism with multiple sclerosis in Turkish population
Authors: Muhsin Elmas , Mustafa Solak , Mehmet Yaman , Saliha Handan Yildiz , Mujgan Erdogan , Tulay Koken
Article Info
Authors
Muhsin Elmas
Department of Medical Genetics, Afyonkarahisar Health Sciences University, Western Anatolia, Turkey
Mustafa Solak
Department of Medical Genetics, Afyonkarahisar Health Sciences University, Western Anatolia, Turkey
Mehmet Yaman
Department of Neurology, Afyon Kocatepe University, Western Anatolia, Turkey
Saliha Handan Yildiz
Department of Medical Genetics, Afyonkarahisar Health Sciences University, Western Anatolia, Turkey
Mujgan Erdogan
Department of Medical Genetics, Afyonkarahisar Health Sciences University, Western Anatolia, Turkey
Tulay Koken
Department of Medical Genetics, Afyonkarahisar Health Sciences University, Western Anatolia, Turkey
Publication History
Received: February 19, 2020
Revised: March 12, 2020
Accepted: April 07, 2020
Published: May 14, 2020
Abstract
Background: Multiple sclerosis (MS) is a demyelinating disease in which the insulating covers of nerve cells in the brain and spinal cord are damaged. In this study, we investigated the possible association of vitamin D levels and rs703842 in the CYP27B1 gene with MS. Methodology: We used blood samples of 99 patients (65 female, 35 male) with an magnetic resonance imaging (MRI)-confirmed definitive diagnosis of MS and 99 controls (70 female, 29 male) between the ages of 18-55 years. We measured their vitamin D levels, isolated their DNA, and scanned rs703842 polymorphism in the CYP27B1 gene. Results: Rs703842 polymorphism in the CYP27B1 gene in humans was found as three different genotypes: CC, CT, and TT. Among them, CC genotype was found higher in the patient group and CT genotype was found higher in the control group. The statistical analysis showed that the probability of a C allele having an association with MS to be 1.5189 times of the probability of T allele. Also, the vitamin D levels in the patient group were detected lower than the control group. Conclusion: Low levels of vitamin D and low expression of CYP27B1 were found to have an association with MS.
Keywords: Multiple sclerosis, vitamin D, 25-Hydroxyvitamin D3 1-alpha-Hydroxylase
